PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis
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چکیده
منابع مشابه
An Evaluation of Frankliniella occidentalis (Thysanoptera: Thripidae) and Frankliniella intonsa (Thysanoptera: Thripidae) Performance on Different Plant Leaves Based on Life History Characteristics
To compare the performance of Frankliniella occidentalis (Pergande) and native Frankliniella intonsa (Trybom) on cucumber and tomato leaves in laboratory, life history characters were investigated, and life tables were constructed using the method of age-stage, two-sex table life. Compared with tomato leaf, there were shorter total preoviposition period (TPOP), higher fecundity, longer female l...
متن کاملAnalysis of the Salivary Gland Transcriptome of Frankliniella occidentalis
Saliva is known to play a crucial role in insect feeding behavior and virus transmission. Currently, little is known about the salivary glands and saliva of thrips, despite the fact that Frankliniella occidentalis (Pergande) (the western flower thrips) is a serious pest due to its destructive feeding, wide host range, and transmission of tospoviruses. As a first step towards characterizing thri...
متن کاملEvaluation and Validation of Reference Genes for qRT-PCR Normalization in Frankliniella occidentalis (Thysanoptera:Thripidae)
Quantitative real time PCR (qRT-PCR) has emerged as a reliable and reproducible technique for studying gene expression analysis. For accurate results, the normalization of data with reference genes is particularly essential. Once the transcriptome sequencing of Frankliniella occidentalis was completed, numerous unigenes were identified and annotated. Unfortunately, there are no studies on the s...
متن کاملTaqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine.
Western flower thrip (Frankliniella occidentalis) is a major global pest of agricultural products. It directly damages crops through feeding, oviposition activity or transmission of several plant viruses. We describe a Taqman real-time quantitative PCR detection system, which can rapidly identify F. occidentalis from thrips larvae to complement the traditional morphological identification. The ...
متن کاملDevelopment and Characterization of 18 Novel EST-SSRs from the Western Flower Thrips, Frankliniella occidentalis (Pergande)
The western flower thrips, Frankliniella occidentalis (Pergande), is an invasive species and the most economically important pest within the insect order Thysanoptera. For a better understanding of the genetic makeup and migration patterns of F. occidentalis throughout the world, we characterized 18 novel polymorphic EST-derived microsatellites. The mutational mechanism of these EST-SSRs was al...
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ژورنال
عنوان ژورنال: Journal of Plant Protection Research
سال: 2016
ISSN: 1899-007X
DOI: 10.1515/jppr-2016-0009